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Merck
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U8501

Sigma-Aldrich

尿苷-5′-二磷酸葡萄糖焦磷酸化酶 来源于面包酵母

Type X, lyophilized powder, ≥40 units/mg protein

别名:

UTP:α-D-葡萄糖-1-磷酸尿苷酰基转移酶

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About This Item

CAS号:
9026-22-6
EC 号:
2.7.7.9 (BRENDA, IUBMB)
EC號碼:
232-811-2
MDL號碼:
MFCD00132561
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bakers yeast

品質等級

200

種類

Type X

形狀

lyophilized powder

比活性

≥40 units/mg protein

成份

Protein, 30-60% modified Warburg-Christian

異物活動

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

儲存溫度

−20°C

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一般說明

尿苷-5′-二磷酸葡萄糖焦磷酸化酶（UDP-glc-PPase）普遍存在于植物、酵母、细菌和哺乳动物中。这种酶是由八个相同亚基组成的八聚体。

應用

来自面包酵母的尿苷-5′-二磷酸葡萄糖焦磷酸化酶′已用于：
  • 合成尿苷-5′-二磷酸葡萄糖(UDP-Glc)-13C9
  • 量化水稻中蔗糖合酶（SUS）的活性
  • 研究己糖激酶和糖原合成酶控制青蛙卵母细胞通量的作用

生化／生理作用

尿苷-5′-二磷酸葡萄糖焦磷酸化酶（UDP-glc-PPase）参与催化UDP-葡萄糖的合成。这种酶的活性需要二价阳离子，例如Mg2+、Ca2+、Mn2+、Ni2+。

單位定義

一个单位将在25℃和pH 7.6下，每分钟由尿苷-5′-二磷酸葡萄糖和无机焦磷酸盐形成1.0 μmole的1-磷酸葡萄糖。

外觀

冻干粉，不含硫酸盐，含柠檬酸缓冲盐

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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