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Merck

T7659

Sigma-Aldrich

胰蛋白酶抑制剂,确定的(1X)溶液

Animal component free, BioReagent, suitable for cell culture

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.75

無菌

sterile-filtered

產品線

BioReagent

形狀

solution

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−20°C

應用

用于中和胰蛋白酶/EDTA溶液的作用。
设计用于角质形成细胞传代培养的本品含有Kunitz型大豆胰蛋白酶抑制剂(SBTI),可抑制丝氨酸蛋白酶(如胰蛋白酶和类胰蛋白酶)的催化活性。 它用于细胞培养以终止胰蛋白酶的作用,胰蛋白酶用于在传代过程中从基质中释放细胞。 它也可能用于其他抗丝氨酸蛋白酶研究。

外觀

溶于98.7% DPBS和1.3%大豆胰蛋白酶抑制剂中。

重構

以1:1 TID:胰蛋白酶/EDTA的最小体积比使用。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Sarra Djemil et al.
Cellular and molecular neurobiology, 41(8), 1787-1799 (2020-08-30)
Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer's disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological loss
I B Svendsen et al.
Carlsberg research communications, 54(6), 231-239 (1989-01-01)
A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined
Maurizio Trovato et al.
Biochemical and biophysical research communications, 302(2), 311-315 (2003-02-27)
The design of minimal units required for enzyme inhibition is a major field of interest in structural biology and biotechnology. The successful design of the cyclic dodecapeptide corresponding to the Phe17-Val28 reactive site amino acid sequence of the low-molecular-mass trypsin
C L Dumke et al.
Journal of applied physiology (Bethesda, Md. : 1985), 92(2), 657-664 (2002-01-18)
Serum proteins [molecular weight (MW) > 10,000] are essential for increased insulin-stimulated glucose transport after in vitro muscle contractions. We investigated the role of the kallikrein-kininogen system, including bradykinin, which is derived from kallikrein (MW > 10,000)-catalyzed degradation of serum

实验方案

天然胰蛋白酶抑制剂也称为丝氨酸蛋白酶抑制剂(serpins),是最大和最多样化的蛋白酶抑制剂家族。Serpins通过抑制体内丝氨酸蛋白酶来控制蛋白质的活化和分解代谢。

Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.

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