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生物源
simian virus
重組細胞
expressed in insect cells
化驗
≥80% (SDS-PAGE)
形狀
frozen liquid
分子量
~83.3 kDa
包裝
pkg of 5 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
400 μg/mL
顏色
clear colorless
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
simian virus ... SV40gp6(1489531)
生化／生理作用
The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.
The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.
外觀
Clear and colorless frozen liquid solution
準備報告
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Z Q Pan et al.
The Journal of biological chemistry, 271(36), 22111-22116 (1996-09-06)
The replication of simian virus 40 (SV40) DNA in vitro requires a trimeric single-stranded DNA (ssDNA)-binding protein called HSSB or RPA. HSSB supports the unwinding of DNA containing the SV40 origin in the presence of the viral-encoded T antigen and
Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.
Dornreiter I
The Embo Journal, 11, 769-776 (1992)
Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen.
Ewald D
Science, 273, 1384-1386 (1996)
H H Chao et al.
Molecular and cellular biology, 20(20), 7624-7633 (2000-09-26)
At least three domains of simian virus 40 large T antigen (TAg) participate in cellular transformation. The LXCXE motif of TAg binds to all members of the retinoblastoma protein (pRB) family of tumor suppressors. The N-terminal 70 residues of TAg
Simian virus 40 large T antigen: the puzzle, the pieces, and the emerging picture.
E Fanning
Journal of virology, 66(3), 1289-1293 (1992-03-01)
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