SRP0183

Hsp90b Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• 别名: HSP84, HSP90Beta, HSPC2, Heat shock protein 90 kDa β
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥80% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 83 kDa
• 包裝: pkg of 200 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: AY359878
• UniProt登錄號: P08238
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... HSP90AB1(3326)

说明

一般說明

Hsp90b (heat shock protein 90-β) is a constitutive cytosolic isoform of Hsp90. The gene is mapped to human chromosome 6p21.1. Hsp90b is an ATP-dependent molecular chaperone and is upregulated in many tumor cells.
Human Heat Shock Protein 90β (GenBank Accession No. AY359878), full length with C-terminal His tag, MW = 83kDa, expressed in an Escherichia coli expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化／生理作用

Hsp90 (heat shock protein 90) is needed for folding, maturation, transport, and degradation of various proteins, particularly signaling molecules, steroid receptors, and transcription factors. Hsp90b (Hsp90 β) is secreted by various cells and works as a chaperone for selected surface-associated or secreted proteins. It acts as a chaperone for matrix metalloproteinases and extracellular matrix proteins, and helps in cell motility. Suppression of the Hsp90b activity is suggested as a therapeutic approach for various cancers and inflammatory diseases.

外觀

Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1