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Merck

SAE0045

Sigma-Aldrich

HRV-3C 蛋白酶

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

别名:

Levlfqgp 站点蛋白酶, 人鼻病毒 3C 蛋白酶, 预断蛋白酶

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

human (human Rhinovirus Type 14)

品質等級

化驗

≥90% (SDS-PAGE)

形狀

aqueous solution

比活性

≥5000 U/mg

分子量

21 kDa

濃度

0.8-1.2 mg/mL

技術

protein purification: suitable

適合性

suitable for protein modification

應用

life science and biopharma

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

来自人鼻病毒 14 类型的 HRV-3C 蛋白酶是一种在 8 个残基识别序列内特异性切割的蛋白酶。
蛋白水解裂解发生在 Gln 和 Gly 残基之间。
HRV-3C 蛋白酶是一种有用的工具,用于切割在载体结构域和目的蛋白之间以该序列作为融合蛋白表达的重组蛋白。
该重组版本含有 6 个组氨酸标签,可通过 IMAC 色谱法轻松去除。

應用

HRV-3C 蛋白酶已被用于去除 DsCystatin 蛋白上的 GST 标签。

單位定義

一单位HRV-3C蛋白酶定义为:在0°C条件下,在反应缓冲液中每小时消化1 nM底物肽(H-Glu-Ala-Leu-Phe-Gln-pNA)所需的酶量。反应缓冲液组成为25 mM HEPES(pH 7.5)、150 mM NaCl、1 mM EDTA和1 mM DTT。

外觀

以溶液形式提供,含 50 mM Tris HCl(pH 7.5)、0.15 MNaCl、1 mM TCEP 和 50% (V/V) 甘油。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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Micah Rapp et al.
Cell reports, 35(1), 108950-108950 (2021-04-02)
Antibodies with heavy chains that derive from the VH1-2 gene constitute some of the most potent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies yet identified. To provide insight into whether these genetic similarities inform common modes of recognition, we
Zhengmao Xu et al.
Parasites & vectors, 12(1), 341-341 (2019-07-13)
Rhipicephalus haemaphysaloides is a widespread tick species in China and other South East Asian countries, where it is the vector of many pathogens. The objective of this study was to study the role of serpin (serine protease inhibitor) during the
Richard W Birkinshaw et al.
Molecular cell, 81(10), 2123-2134 (2021-04-02)
A body of data supports the existence of core (α2-α5) dimers of BAK and BAX in the oligomeric, membrane-perturbing conformation of these essential apoptotic effector molecules. Molecular structures for these dimers have only been captured for truncated constructs encompassing the
Activity of the human rhinovirus 3C protease studied in various buffers, additives and detergents solutions for recombinant protein production
Raheem U et al.
PLoS ONE (2016)
An immunosuppressive tick salivary gland protein DsCystatin interferes with Toll-like receptor signaling by downregulating TRAF6
Ta S et al.9
Frontiers in Immunology, 9 (2018)

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Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

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