SAB4200337

Anti-phospho-RasGRP1(pThr¹⁸⁴) antibody, Mouse monoclonal

clone p-RasGRP1-1, purified from hybridoma cell culture

• 别名: Anti-CALDAG-GEFI, Anti-CALDAG-GEFII, Anti-RAS guanyl releasing protein 1 (calcium and DAG-regulated) hRasGRP1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: p-RasGRP1-1, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~90 kDa
• 物種活性: human
• 濃度: ~1.0 mg/mL
• 技術: immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human RasGRP1
• 同型: IgG2a
• UniProt登錄號: O95267
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pThr184)
• 基因資訊: human ... RASGRP1(10125) ; mouse ... Rasgrp1(19419) ; rat ... Rasgrp1(29434)

说明

一般說明

Monoclonal Anti-phospho-RasGRP1(pThr184) (mouse IgG2a isotype) is derived from the hybridoma p-RasGRP1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1 conjugated to KLH. RAS guanyl releasing protein 1 (RasGRP1) is a member of RasGRP family, characterized by the presence of a N-terminal Ras superfamily guanine nucleotide exchange factor (GEF) domain and and C-terminal calcium- and diacylglycerol (DAG)/phorbol ester-binding domains. It is an intracellular signaling protein. RasGRP1 has a N terminal guanine nucleotide exchange factor domain for Ras-like guanosine triphosphatases (GTPases) (RasGEFN/RasGEF), two EF hand motifs and a carboxy-terminal C1 domain.

免疫原

a synthetic phospho-peptide corresponding to residues surrounding Thr¹⁸⁴ of human RasGRP1, conjugated to KLH. The corresponding sequence is identical in rat, mouse, monkey, bovine and canine RasGRP1.

應用

Monoclonal Anti-phospho-RasGRP1(pThr184) antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

生化／生理作用

RAS guanyl releasing protein 1 (RasGRP1) functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound guanosine diphosphate (GDP) for guanosine triphosphate (GTP). It activates the extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable