推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
10.1, monoclonal
物種活性
human
技術
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... RASGRP1(10125)
一般說明
RasGRP1, also known as Calcium and DAG-regulated guanine nucleotide exchange factor II (CalDAG-GEFII) or Ras guanyl-releasing protein (RASGRP), and encoded by the gene RASGRP1/RASGRP, functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. RasGRP1 activates the Erk/MAP kinase cascade and couples T-lymphocytes and B-lymphocytes antigen receptors to the activation of Ras. Hence, it regulates T-cells and B-cells development, homeostasis and differentiation. Additionally, RasGRP1 functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating allergic responses. RasGRP1 may also function in other cell types’ differentiation. RasGRP1 is localized to the cytoplasm and cell membrane; it is a peripheral membrane protein in the Golgi/ER membranes. In neurons, RasGRP1 localizes to somata and dendrites but not to axons of hippocampal pyramidal cells of in the brain and in particular the cerebellum, cortex and amygdata. RasGRP1 also is expressed in the hematopoietic system including T cells and RasGRP1 is also found expressed developmentally in fetal brain and kidney tissues. Defects in RasGRP1 may be associated with Systemic lupus erythematosus (SLE) a chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Interestingly it is thought that aberrantly spliced isoforms or other mutations of RASGRP1 give rise to dysregulation of various signaling processes which in turn contribute to the development of autoimmunity in SLE patients.
免疫原
GST-tagged recombinant protein corresponding to human RasGRP1.
應用
Detect RasGRP1 using this mouse monoclonal antibody, Anti-RasGRP1 Antibody & clone 10.1 validated for use in western blotting.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected RasGRP1 in 10 µg of human cerebellum tissue lysate.
Flow Cytometry Analysis: A representative lot detected RasGRP1 in malignant lymphoid cells treated with tipifarmib (Ding, H., et al. (2011). Blood. 118:4872-4881).
Flow Cytometry Analysis: A representative lot detected RasGRP1 in malignant lymphoid cells treated with tipifarmib (Ding, H., et al. (2011). Blood. 118:4872-4881).
品質
Evaluated by Western Blotting in MOLT-4 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RasGRP1 in 10 µg of MOLT-4 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RasGRP1 in 10 µg of MOLT-4 cell lysate.
標靶描述
~90 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~62 kDa, ~55 kDa, and ~68 kDa
外觀
Format: Purified
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Arianna De Rosa et al.
Biomolecules, 12(2) (2022-02-26)
Schizophrenia (SCZ) is a polygenic severe mental illness. Genome-wide association studies (GWAS) have detected genomic variants associated with this psychiatric disorder and pathway analyses have indicated immune system and dopamine signaling as core components of risk in dorsolateral-prefrontal cortex (DLPFC)
Neelam Shahani et al.
Science signaling, 9(454), ra111-ra111 (2016-12-03)
The striatum of the brain coordinates motor function. Dopamine-related drugs may be therapeutic to patients with striatal neurodegeneration, such as Huntington's disease (HD) and Parkinson's disease (PD), but these drugs have unwanted side effects. In addition to stimulating the release
Sarah Winter et al.
EMBO molecular medicine, 10(2), 188-199 (2017-12-29)
Inherited CTPS1, CD27, and CD70 deficiencies in humans have revealed key factors of T-lymphocyte expansion, a critical prerequisite for an efficient immunity to Epstein-Barr virus (EBV) infection. RASGRP1 is a T-lymphocyte-specific nucleotide exchange factor known to activate the pathway of
Mehdi Eshraghi et al.
Science advances, 6(18), eaaz7001-eaaz7001 (2020-05-20)
The therapeutic effects of l-3,4-dihydroxyphenylalanine (l-DOPA) in patients with Parkinson's disease (PD) severely diminishes with the onset of abnormal involuntary movement, l-DOPA-induced dyskinesia (LID). However, the molecular mechanisms that promote LID remain unclear. Here, we demonstrated that RasGRP1 [(guanine nucleotide
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