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Merck

S4942

Supelco

SYPRO ® 红宝石蛋白凝胶染色剂

别名:

SYPRO® dye, protein gel stain

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About This Item

分類程式碼代碼:
41105322
NACRES:
NA.32

儲存期限

≥6 mo. (when stored at room temperature and protected from light)

技術

protein staining: suitable

螢光

λex 280,450 nm; λem 610 nm

一般說明

SYPRO Ruby 蛋白凝胶染色剂是一种即用型、超灵敏的发光染色剂,用于检测聚丙烯酰胺凝胶电泳 (PAGE) 分离的蛋白。该染色剂专为 2-D PAGE 设计,已被证明是 1-D PAGE 和等电聚焦 (IEF) 凝胶的极佳选择。SYPRO 红宝石蛋白凝胶染色的灵敏度与许多银染技术相当。但是,与银染不同的是,SYPRO Ruby 凝胶染色:
  • 使用简单的染色方案,无过度染色的可能性
  • 提供超过三个数量级的线性定量范围
  • 显示较少的蛋白质间变异性
  • 染色糖蛋白、脂蛋白、钙结合蛋白、纤维状蛋白和其他难染色蛋白
  • 不会染色外源性核酸
  • 不会干扰基于 Edman 测序或质谱的蛋白质后续分析

應用

SYPRO 红宝石蛋白凝胶染色剂用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 后的蛋白染色。

注意

避光保存。

法律資訊

SYPRO is a registered trademark of Life Technologies

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Filipe Natalio et al.
Cell and tissue research, 339(2), 429-436 (2009-12-17)
Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations
Chronic hypoxia alters mitochondrial composition in human macrophages.
Fuhrmann DC, et al.
Biochimica et Biophysica Acta, 1834, 2750-2760 (2013)
Christoph Schröder et al.
Methods in molecular biology (Clifton, N.J.), 785, 203-221 (2011-09-09)
Antibody microarrays are a multiplexing technique for the analyses of hundreds of different analytes in parallel from small sample volumes of few microlitres only. With sensitivities in the picomolar to femtomolar range, they are gaining importance in proteomic analyses. These
Mario Navarrete et al.
Clinical proteomics, 10(1), 17-17 (2013-11-19)
Serine hydrolases constitute a large enzyme family involved in a diversity of proteolytic and metabolic processes which are essential for many aspects of normal physiology. The roles of serine hydrolases in renal function are largely unknown and monitoring their activity
Jun Zou et al.
eLife, 4, e09406-e09406 (2015-10-17)
Truncating mutations in the giant sarcomeric protein Titin result in dilated cardiomyopathy and skeletal myopathy. The most severely affected dilated cardiomyopathy patients harbor Titin truncations in the C-terminal two-thirds of the protein, suggesting that mutation position might influence disease mechanism.

商品

为满足蛋白分析的多样化需求,Sigma提供多种蛋白质可视化(染色)试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计,即使在传统的PAGE形式中依然表现出色。

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

实验方案

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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