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Merck
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主要文件

RES3098T-B7

SAFC

TRIS盐酸盐

别名:

Trizma® 盐酸盐 盐酸盐, TRIS 盐酸盐, 三羟基甲基氨基甲烷 盐酸盐, 三羟甲基氨基甲烷盐酸盐, 盐酸三甲胺

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About This Item

线性分子式:
NH2C(CH2OH)3 · HCl
CAS号:
分子量:
157.60
Beilstein:
3675235
EC號碼:
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.21

生物源

synthetic

形狀

powder

技術

cell culture | mammalian: suitable

雜質

Endotoxin, microbial, and trace metals, tested

有用的pH值範圍

7.0-9.0

pKa (25 °C)

8.1

適合性

suitable for manufacturing use

異物活動

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES 字串

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

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應用

TRIS盐酸盐是一种常用的生物缓冲液,又称为Trizma HCl。 Tris缓冲液被广泛用于诊断、下游纯化和细胞培养等生物系统中。Tris的pKa为7.77,与生理pH值7.36非常接近。

Tris HCl被用于生物制药缓冲液制剂(上游和下游)、电泳分离和诊断试剂配方。Tris HCl特别适用于下游纯化过程。

包裝

本品具有以下包装规格:
RES3098T-B701X: 100g容器
RES3098T-B703X: 5kg容器
RES3098T-B703X: 25kg容器
RES3098T-B703X: 50kg容器

其他說明

所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液,温度每降低 1°C,pH 就会增加约 0.03 单位,每稀释 10 倍,pH 会降低 0.03-0.05 单位。对于一些精准的应用,请使用经过仔细校准的pH计和玻璃/甘汞组合电极。

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

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