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等级
Molecular Biology
for molecular biology
表单
buffered aqueous glycerol solution
浓度
10,000 units/mL
运输
wet ice
储存温度
−20°C
特异性
Recognition sequence: 5′-GmA/TC-3′
Ligation and recutting results: After 2-10-fold Dpn I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Dpn I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75 °C for 15 minutes.
应用
DpnI is a restriction endonuclease that is used in molecular biological applications to cleave the recognition sequence 5′-GmA/TC-3′, generating DNA framents with blunt ends.
其他说明
Supplied with 10x Restriction Enzyme Buffer SA (B7531).
Comment: Since Dpn I will completely cleave only fully methylated pBR322 DNA, cleavage of 95% or more is considered complete digestion.
外形
Solution in 10 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol (v/v) at 4 °C
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
S Lacks et al.
The Journal of biological chemistry, 250(11), 4060-4066 (1975-06-10)
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The Journal of biological chemistry, 278(15), 12769-12778 (2003-02-01)
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Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
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Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
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