PP2400

Bacterial GST Tag Vector Set

plasmid vectors for molecular cloning

• 别名: cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• 分類程式碼代碼: 12352200
• NACRES: NA.85

属性

• 籤條: GST tagged
• 形狀: buffered aqueous solution
• 菌種選擇: kanamycin
• 複製起點: pUC (500 copies)
• 肽切割: TEV; no cleavage
• 肽標籤位置: C-terminal; N-terminal
• 啟動子: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

Molecular cloning often benefits from optimizing the vector used for expression.

This pack enables you to compare placing Glutathione-S-Transferase (GST) affinity/solubility tags at either the N or C terminus of your gene of interest (inserted into the MCS, under transcriptional control of the OXB20 strong bacterial promoter) with, and also without a TEV (Tobacco Etch Virus) protease cleavage site. The TEV site enables removal of the GST tag from the protein following production. Comparing these four configurations should enable you to evaluate how best to express and detect your gene of interest from bacterial cells. We also provide many other functional tags and cleavage sites, if required.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.

Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.

Transcription Termination: These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

序列

For Genebank sequence, FASTA sequence, Quick-reference Plasmid Map, and Full Plasmid Map, see the individual vector product page for links.

分析報告

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

其他說明

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics^™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

法律資訊

These plasmids are sold free of reach-through rights and can be used to make commercial products. However the plasmids themselves (or derivatives) cannot be sold.

Oxford Genetics is a trademark of Oxford Genetics Ltd

试剂盒组分也可单独购买

• PSF-OXB20-NH2-GST-TEV - N-TERMINAL GST TAG BACTERIAL PLASMID, plasmid vector for molecular cloning
• OGS3182 PSF-OXB20-COOH-GST - C-TERMINAL GST TAG BACTERIAL PLASMID, plasmid vector for molecular cloning
• PSF-OXB20-NH2-GST - N-TERMINAL GST TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable