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籤條
FLAG® tagged
形狀
buffered aqueous solution
分子量
size 6173 bp
菌種選擇
kanamycin
哺乳動物細胞選擇
puromycin
複製起點
pUC (500 copies)
肽切割
no cleavage
肽標籤位置
N-terminal
啟動子
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
報告基因
none
運輸包裝
ambient
儲存溫度
−20°C
一般說明
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
序列
To view sequence information for this product, please visit the product page
分析報告
To view the Certificate of Analysis for this product, please visit www.oxgene.com
法律資訊
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
相關產品
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Tomomi Yamamoto-Fukuda et al.
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 272(10), 2689-2696 (2014-08-21)
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The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
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