跳转至内容
Merck
所有图片(1)

文件

F4799

Sigma-Aldrich

3xFLAG™ Peptide

≥90% (HPLC/MS), lyophilized powder

别名:

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk肽, dykddddk肽

登录查看公司和协议定价
所有图片(1)

About This Item

经验公式（希尔记法）:
C120H169N31O49S
分子量:
2861.87
MDL號碼:
MFCD01863911
分類程式碼代碼:
12352202
NACRES:
NA.32

一般說明

3X FLAG肽是一种具有23个氨基酸残基的合成肽。在该肽中Asp-Tyr-Lys-Xaa-Xaa-Asp基序重复了三次。在C-末端的八个氨基酸组成了经典的FLAG序列(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)。
Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.

應用

用于从在溶液中的或结合至抗-FLAG® M2琼脂糖亲和凝胶（A2220）上琼脂糖上的抗-FLAG ®M2单克隆抗体（F1804）进行3X FLAG®融合蛋白的竞争性洗脱。通过亲和色谱法可实现这一目标。用于将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上温和的洗脱。1X FLAG肽（F3290）将不会把3X FLAG融合蛋白从抗-FLAG®亲和凝胶上洗脱下来。


请访问我们的FLAG® 应用 门户网站，了解更多产品详情。

準備報告

如需制备储备溶液，可溶解于TBS（含有150 mM NaCl的50mM Tris-HCl，pH7.4）至5 mg/mL的浓度。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Yannick Doyon et al.
Molecular and cellular biology, 24(5), 1884-1896 (2004-02-18)
The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost
Paul L Colbert et al.
Oncotarget, 6(2), 953-968 (2014-12-02)
Microtubules (MTs) are components of the cytoskeleton made up of polymerized alpha and beta tubulin dimers. MT structure and function must be maintained throughout the cell cycle to ensure proper execution of mitosis and cellular homeostasis. The protein tyrosine phosphatase
Andrew T Schiffmacher et al.
The Journal of cell biology, 215(5), 735-747 (2016-11-20)
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest cells reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, to permit their EMT and migration. Serial processing of Cad6B by a
Min Hwa Shin et al.
PLoS genetics, 12(2), e1005884-e1005884 (2016-03-02)
The inactivation of p53 creates a major challenge for inducing apoptosis in cancer cells. An attractive strategy is to identify and subsequently target the survival signals in p53 defective cancer cells. Here we uncover a RUNX2-mediated survival signal in p53
Lingyan Wang et al.
Nature communications, 8, 13876-13876 (2017-02-09)
Cellular protein interaction networks are integral to host defence and immune signalling pathways, which are often hijacked by viruses via protein interactions. However, the comparative virus-host protein interaction networks and how these networks control host immunity and viral infection remain
查看所有相关文献

商品

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Anti-FLAG® M2磁珠

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

实验方案

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀

采用M2单克隆抗体4％琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀（IP）实验方案

相关内容

Protein Purification

Protein purification techniques, reagents, and methods for recombinant protein purification, including ion exchange, exclusion chromatography, and protein affinity chromatography.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

蛋白表达

蛋白表达技术，可在大肠杆菌、昆虫、酵母和哺乳动物的表达系统中表达重组蛋白，用于基础研究、研发治疗方法及协助疫苗生产。

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门