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F2426

EZview^™ Red 抗-FLAG^® M2 亲和凝胶

Name: EZview<SUP>™</SUP> Red 抗-FLAG<SUP>®</SUP> M2 亲和凝胶
Brand: SIGMA

clone M2

别名:

单克隆抗-FLAG^® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:

12352203

NACRES:

NA.32

無性繁殖:

M2, monoclonal

application:

IP
affinity chromatography

技術:

affinity chromatography: suitable
immunoprecipitation (IP): suitable

citations:

204

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無性繁殖

M2, monoclonal

品質等級

200

分析物化學類別

proteins

技術

affinity chromatography: suitable
immunoprecipitation (IP): suitable

基質

4% agarose bead; 45-165μm bead size

同型

IgG1

容量

≥0.6 mg/mL, gel binding capacity

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

EZview Red 抗-FLAG M2 亲和凝胶是含有抗FLAG M2抗体的树脂，共价交联在4%的琼脂糖珠上。该亲和凝胶用于将标记融合蛋白与样品结合，如细胞裂解物和组织，用于纯化标记蛋白，用于制备免疫沉淀分析。红色染料增强了可视性，以获得更有效的结果。琼脂糖珠结合于N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

特異性

适用于纯化N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

應用

FLAG-和3xFLAG-标签融合蛋白的免疫沉淀（IP）。

洗脱-FLAG 多肽，甘氨酸，pH 3.5，3x FLAG 多肽

在我们的 FLAG^® 应用门户了解更多产品详情。

外觀

1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

EZview is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

FLAG™ 亲和凝胶，FLAG™标签，3xFLAG™标签，DYKDDDDK标签

06693

Timestrip Plus^™ -20 °C温度指示标签

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Radiosensitization of MDA-MB-231 breast tumor cells by adenovirus-mediated overexpression of a fragment of the XRCC4 protein.

Kara R Jones et al.

Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)

Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to

The RING finger domain of Varicella-Zoster virus ORF61p has E3 ubiquitin ligase activity that is essential for efficient autoubiquitination and dispersion of Sp100-containing nuclear bodies.

Matthew S Walters et al.

Journal of virology, 84(13), 6861-6865 (2010-04-16)

Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity

The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response.

Jiahai Zhou et al.

Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)

The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I

Severe acute respiratory syndrome coronavirus papain-like protease ubiquitin-like domain and catalytic domain regulate antagonism of IRF3 and NF-kappaB signaling.

Matthew Frieman et al.

Journal of virology, 83(13), 6689-6705 (2009-04-17)

The outcome of a viral infection is regulated in part by the complex coordination of viral and host interactions that compete for the control and optimization of virus replication. Severe acute respiratory syndrome coronavirus (SARS-CoV) intimately engages and regulates the

Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain.

Annie M Sriramachandran et al.

Nature communications, 10(1), 3678-3678 (2019-08-17)

Modification with SUMO regulates many eukaryotic proteins. Down-regulation of sumoylated forms of proteins involves either their desumoylation, and hence recycling of the unmodified form, or their proteolytic targeting by ubiquitin ligases that recognize their SUMO modification (termed STUbL or ULS).

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实验方案

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀

采用M2单克隆抗体4％琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀（IP）实验方案

主要文件

EZview^™ Red 抗-FLAG^® M2 亲和凝胶