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Merck

MBD0056

Sigma-Aldrich

Trace Elements Ready Made Solution

100X, microbial growth medium supplement

别名:

micronutrient

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About This Item

分類程式碼代碼:
41106212
NACRES:
NA.54

品質等級

無菌

0.2 μm filtered

形狀

liquid

儲存期限

2 yr

包裝

pkg of 50 mL

儲存條件

protect from light

濃度

100 ×

適合性

suitable for (Media supplement)

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

Minerals and vitamins are broadly used to supplement growth media in order to support growth of a variety of different bacteria. The growth media mimics the natural environment of the bacteria thus providing an optimal setting for high throughput cultures. Trace elements ready-made solution, MBD0056, is comprised of vital minerals to support critical bacterial development. The solution is based on Wolin and Wolfe’s recipe, which is especially suitable for culturing anaerobic bacteria of the human microbiome ( )

特點和優勢

  • 0.2uM filtered for removal of contaminants
  • Saves time in preparation of media
  • Improved accuracy: composition and concentrations are analyzed and established by inductively coupled plasma (ICP)
  • Ready-made liquid mixture containing all ingredients.
  • Decreases exposure to chemical and toxic risks associated with the powder form of the trace elements

成分

50 mL Trace elements solution:
  • EDTA : 570 mg/L
  • CuSO4. 5H2O :10 mg/L
  • AlK(SO4)2 · 12H2O : 20 mg/L
  • H3BO3 :10 mg/L
  • Na2MoO4. 2H2O : 10 mg/L
  • Na2SeO3 : 1 mg/L
  • Na2WO4 . 2H2O : 10 mg/L
  • NiCl2 . 6H2O : 20 mg/L
  • MgSO4 . 7H2O : 3000 mg/L
  • MnSO4 . H2O : 500 mg/L
  • NaCl : 1000 mg/L
  • NH4Fe(SO4)2 · 12H2O :170 mg/L
  • Co(NO3)2 . 6H2O : 100 mg/L
  • CaCl2 · 2H2O :100 mg/L
  • ZnSO4 . 7H2O :100 mg/L

其他說明

Add to pre-sterilized and cooled growth media. Concentration 100x.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Methanogens: reevaluation of a unique biological group
Balch, W. E., et al.
Microbiological Reviews, 43(2), 260-296 (1979)
Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice
Goodman, Andrew L., et al.
Proceedings of the National Academy of Sciences of the USA, 108(15), 6252-6257 (2011)
Viologen dye inhibition of methane formation by Methanobacillus omelianskii
Wolin, E. A., R. S. Wolfe, and M. J. Wolin
Journal of Bacteriology, 87(5), 993-998 (1964)
Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria
Ito, Tamaki, et al.
Gut Microbes, 10.1, 77-91 (2019)

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