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用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
neurological disorders; cancer
儲存溫度
−20°C
基因資訊
human ... PDHA1(5160) , PDHA2(5161) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)
一般說明
丙酮酸脱氢酶（PDH）是一种线粒体酶，它可以催化丙酮酸转化为乙酰辅酶a和一氧化碳2，也可以连接三羧酸（TCA）和糖酵解路径。该酶被磷酸化抑制，被去磷酸化激活。PDH突变与丙酮酸脱氢酶缺乏（导致乳酸酸中毒和神经功能障碍）和莱氏症候群有关。 PDH也与癌基因诱导的衰老有关。 PDH测量可以为代谢功能和肿瘤发生提供参考。
應用
PDH活性测定试剂盒已用于测定丙酮酸脱氢酶的活性。
適合性
适于测定不同生物样品内的PDH活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原則
丙酮酸脱氢酶活性可以通过偶联酶反应进行测定，生成的比色产物(450 nm)和酶促活性成正比。一个单位的丙酮酸脱氢酶是在37°C下，每分钟产生 1.0毫克分子量NADH的酶的量。
仅试剂盒组分
产品编号
说明
- PDH Assay Buffer
- PDH Substrate
- PDH Developer
- NADH Standard
- PDH Positive Control
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
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