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Merck

M7195

Sigma-Aldrich

Anti-MO25 (C-terminus) 兔抗

enhanced validation

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-CAB39, Anti-CGI-66, Anti-Calcium binding protein 39

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.44

生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen ~40 kDa

种属反应性

human, mouse (predicted), rat (predicted)

包装

antibody small pack of 25 μL

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

浓度

~2 mg/mL

技术

western blot: 0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... CAB39(51719)
mouse ... Cab39(12283)
rat ... Cab39(301574)

一般描述

MO25 is a 40 kDa scaffolding protein that interacts with STRADα and LKB1 to regulate tumor suppression. MO25 also facilitates the activation and cytoplasmic localization of LKB1 . Anti-MO25 (C-terminus) antibody detects human MO25 (approx. 40 kDa). The product is also predicted to react with rat and mouse MO25. Staining of the MO25 band in immunoblotting is specifically inhibited by the immunizing peptide.

免疫原

synthetic peptide corresponding to amino acids 323-341 located near the C-terminus of human MO25. This sequence is identical in chicken, dog, and bovine MO25 and highly conserved in rat and mouse MO25 (2 amino acid substitutions).

应用

Anti-MO25 (C-terminus) antibody is suitable for use in western blot (0.5-1 μg/mL using HEK-293T cells expressing human MO25 and in PC12 cell lysates).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Elton Zeqiraj et al.
Science (New York, N.Y.), 326(5960), 1707-1711 (2009-11-07)
The LKB1 tumor suppressor is a protein kinase that controls the activity of adenosine monophosphate-activated protein kinase (AMPK). LKB1 activity is regulated by the pseudokinase STRADalpha and the scaffolding protein MO25alpha through an unknown, phosphorylation-independent, mechanism. We describe the structure
Jérôme Boudeau et al.
The EMBO journal, 22(19), 5102-5114 (2003-10-01)
Mutations in the LKB1 protein kinase result in the inherited Peutz Jeghers cancer syndrome. LKB1 has been implicated in regulating cell proliferation and polarity although little is known about how this enzyme is regulated. We recently showed that LKB1 is

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