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Merck

M5310

Sigma-Aldrich

矿物油

light oil, suitable for mouse embryo cell culture, BioXtra

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

sterile-filtered

產品線

BioXtra

形狀

light oil

儲存條件

protect from light

技術

cell culture | embryo: suitable

折射率

n20/D 1.467 (lit.)

密度

0.82-0.88 g/mL at 25 °C

格式

neat

InChI 密鑰

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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一般說明

采用矿物油或液体石油保护各种类型的培养物。矿物油的优点是可降低生长速率,并且几乎完全消除了培养基的水分蒸发。因此,减少了传代培养,培养物活率更高。1914年,矿物油首次用于覆盖细菌培养物。

應用

矿物油已被普遍使用并推荐用于覆盖小鼠胚胎培养物,防止其在培养过程中干燥。
矿物油适用于下列应用:
  • 在细胞活性研究中用于制备培养基或培养基补充剂
  • 通过在阵列周围添加矿物油,防止阵列蒸发
  • 用于制备微量注射针(微量注射针拉伸、锐化和装载)
  • 用于PCR设置

特點和優勢

即用型无菌过滤产品采用便捷的PET瓶包装。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

No data available

閃點(°C)

No data available

個人防護裝備

Eyeshields, Gloves


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Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Slobodan Beronja et al.
Methods in molecular biology (Clifton, N.J.), 961, 351-361 (2013-01-18)
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an

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用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

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