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Merck

M0535

Sigma-Aldrich

用于培养支原体的基础培养基

powder, suitable for microbiology

别名:

Mycoplasma media

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.74

形狀

powder

品質等級

技術

microbiological culture: suitable

溶解度

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

應用

microbiology

一般說明

支原体肉汤是一种基础培养基,在用酵母提取物、马血清和抗生素富集后可支持支原体菌的生长。当用于培养支原体时,该基础培养基中所包含的成分不会表现出抑制或毒性作用。已添加了特殊的矿物质补充剂以改善生物体的生长和菌落特性。

應用

支原体肉汤可用于支原体的分离和培养。它在无氧条件可被用于培养 牙龈卟啉单胞菌

成分

粉末含有(g/L):10.0 细菌蛋白胨、10.0 LAB-LEMCO粉、5.0 氯化钠、0.5 矿物质补充剂。

準備報告

基础培养基:

1.将 25.5 g 培养基粉末溶于 1L 蒸馏水中。搅拌至粉末溶解

2.将溶液的 pH 值调至 7.8 ± 0.2.

3.在 121 °C 和 15 psi 下高温灭菌 15 分钟。冷却至50 °C并添加无菌补充剂。

儲存和穩定性

在室温下储存培养基粉末,在 2-8 °C 制备培养基。

其他說明

此产品仅供实验室使用。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

通过PCR法、DNA染色法和培养法检测细胞培养中的支原体污染。探索我们的支原体预防、清除和检测试剂盒。

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。

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