跳转至内容
Merck
所有图片(1)

文件

MP0035

Sigma-Aldrich

LookOut ® 支原体 PCR 检测试剂盒

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

别名:

PCR-based mycoplasma screening

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

品質等級

100

包裝

pkg of 1 kit

技術

PCR: suitable

應用

detection
microbiology

相容性

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

儲存溫度

2-8°C

一般說明

该LookOut®支原体PCR检测试剂盒利用聚合酶链反应（PCR），这是因其在对细胞培养基中的支原体、无胆甾原体和尿素原体以及其他细胞培养衍生的生物制品的检测中具有最高灵敏度而被确定作为选择的方法。该试剂盒已被成功测试具有对20,000至20个拷贝的发酵支原体基因组DNA进行扩增的能力。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法，整体的检测时间得到了大幅度的下降。

應用

支原体PCR检测试剂盒视频实验方法
LookOut®支原体PCR检测试剂盒已用于支原体感染筛查。

相容性

该试剂盒已被优化与货号D9307一起使用。不推荐任何带有3′-5′外切核酸酶活性的DNA聚合酶。这些包括Tli、Pfu和Pwo。那些可被使用的DNA聚合酶包括Taq、Tfl和Tth。

法律資訊

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

其他客户在看

Slide 1 of 2

1 of 2

双苯并咪唑 H 33258 powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Sigma-Aldrich

B1155

双苯并咪唑 H 33258

SAFC

SAFC

L9042

LookOut ® DNA 擦除酶

Nellie Moshkovich et al.
Cancer research, 80(11), 2125-2137 (2020-04-09)
Peptidylarginine deiminases (PADI) catalyze posttranslational modification of many target proteins and have been suggested to play a role in carcinogenesis. Citrullination of histones by PADI4 was recently implicated in regulating embryonic stem and hematopoietic progenitor cells. Here, we investigated a
Dmitri Pchejetski et al.
Cancer research, 70(21), 8651-8661 (2010-10-21)
Radiotherapy is widely used as a radical treatment for prostate cancer, but curative treatments are elusive for poorly differentiated tumors where survival is just 15% at 15 years. Dose escalation improves local response rates but is limited by tolerance in
Soudabeh Rad Pour et al.
Frontiers in oncology, 10, 51-51 (2020-03-03)
Dysregulation of the kynurenine pathway has been regarded as a mechanism of tumor immune escape by the enzymatic activity of indoleamine 2, 3 dioxygenase and kynurenine production. However, the immune-modulatory properties of other kynurenine metabolites such as kynurenic acid, 3-hydroxykynurenine
Lucian Romeo Farcal et al.
Nanotoxicology, 7(6), 1095-1110 (2012-07-10)
An in vitro human alveolar barrier established by the coculture of epithelial human cell line NCI-H441 with endothelial human cell line ISO-HAS1 was used to evaluate the effects of amorphous silicon dioxide nanoparticles (SiNPs), in the presence or absence of
Jennifer Mary Wymant et al.
Journal of Cancer, 11(11), 3288-3302 (2020-04-02)
Efficacious anticancer therapies for targeting plasma membrane receptors with antibody based therapeutics are often contingent on sufficient endocytic delivery of receptor and conjugate to lysosomes. This results in downregulation of receptor activity and, in the case of antibody-drug conjugates (ADCs)
查看所有相关文献

商品

细胞培养中的支原体检测和清除

通过PCR法、DNA染色法和培养法检测细胞培养中的支原体污染。探索我们的支原体预防、清除和检测试剂盒。

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

胎牛血清 (FBS) – 基础知识

回顾使用胎牛（或小牛）血清进行细胞培养的基础知识，以及采购和法规如何影响细胞测定所用血清的价格。

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

查看所有结果

实验方案

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门