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Merck

L9795

Sigma-Aldrich

乳酸氧化酶 来源于绿色气球菌

lyophilized powder

别名:

L-乳酸单加氧酶, 乳酸加氧酶, 乳酸单加氧酶, 乳酸氧化脱羧酶, 乳酸氧化酶

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Aerococcus viridans)

品質等級

形狀

lyophilized powder

分子量

80 kDa (gel filtration)

儲存溫度

−20°C

一般說明

乳酸氧化酶(LOX)是一种黄素单核苷酸(FMN)依赖型酶,是α-羟酸氧化酶黄素酶(flavoenzyme)家族一员。

應用

来自绿色气球菌 的乳酸氧化酶已被用于:
  • 作为培养基增补剂用于将胞外乳酸转化为丙酮酸
  • 涂布在玻璃珠表面用于以推拉式喷头系统(push-pull nozzle system)进行单个细胞的乳酸电化学检测
  • 涂布在多枝化金纳米颗粒(GNP)表面用于以纸基多路复用设备(multiplexed device)同时比色测定唾液中的乳酸

生化/生理作用

在FMN辅助因子存在的情况下,乳酸氧化酶(LOX)可催化乳酸氧化形成丙酮酸和过氧化氢。该酶通过测定反应中形成的过氧化氢,有助于间接测定L-乳酸。

單位定義

一个单位定义为在标准测定条件下,在 37℃ 下,每分钟产生 1 mmol 过氧化氢的酶量。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Nicholas X Williams et al.
Nature electronics, 4(4), 261-268 (2022-04-05)
Electronic waste can lead to the accumulation of environmentally and biologically toxic materials and is a growing global concern. Developments in transient electronics-in which devices are designed to disintegrate after use-have focused on increasing the biocompatibility, whereas efforts to develop
Ingar Leiros et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 12), 1185-1190 (2006-12-05)
The crystal structure of L-lactate oxidase (LOX) from Aerococcus viridans has been determined at 2.1 A resolution. LOX catalyzes the flavin mononucleotide (FMN) dependent oxidation of lactate to pyruvate and hydrogen peroxide. LOX belongs to the alpha-hydroxy-acid oxidase flavoenzyme family;
Lin Zhou et al.
Analytical chemistry, 93(24), 8680-8686 (2021-06-10)
Metabolism of a single cell, even within the same organization, differs from other cells by orders of magnitude. Single-cell analysis provides key information for early diagnosis of cancer as well as drug screening. Any slight change in the microenvironment may
Cyrielle L Bouchez et al.
STAR protocols, 3(2), 101403-101403 (2022-05-24)
Lactate is a central metabolite in energy metabolism and is also involved in cell signaling and epigenetic regulations. Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range. We detail
Melissa Conti Mazza et al.
Journal of neurochemistry, 162(3), 245-261 (2022-06-18)
Human DJ-1 is a cytoprotective protein whose absence causes Parkinson's disease and is also associated with other diseases. DJ-1 has an established role as a redox-regulated protein that defends against oxidative stress and mitochondrial dysfunction. Multiple studies have suggested that

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