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Merck

GERPN6302

碘代乙酰胺

Cytiva RPN6302, pack of 25 g

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.77

形狀

solid

特點

wetted part: no

包裝

pack of 25 g

製造商/商標名

Cytiva RPN6302

顏色

Yellowish

溶解度

cold water: soluble (Easily soluble in cold and hot water)

儲存溫度

2-8°C

一般說明

Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.

應用

Iodoacetamide has been used:
  • as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
  • in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
  • as a protein alkylation agent

生化/生理作用

Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

特點和優勢

  • Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

象形圖

Health hazard

訊號詞

Danger

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects


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实验方案

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

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