GE17-5478-51

HiTrap^® Protein L

Cytiva 17-5478-51, pack of 5 × 1 mL

• 别名: HiTrap Chromatography Column, Protein L Column
• 分類程式碼代碼: 23151816
• NACRES: NA.56

属性

• ligand: recombinant protein L (E. coli), mammalian free
• 包裝: pack of 5 × 1 mL
• 製造商／商標名: Cytiva 17-5478-51
• 儲存條件: (20% Ehtanol)
• 參數: 5 bar
• 柱內徑: 7 mm
• 基質: rigid, highly cross-linked agarose
• 樣品平均尺寸: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• 工作範圍: 2-10(pH interval where the medium can be operated without significant change in function.)
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

说明

一般說明

HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.

HiTrap^® Protein L columns are packed with Capto^™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto^™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.

應用

HiTrap^® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).

特點和優勢

• Captures a wide range of antibody fragments
• Allows a platform approach for the purification of antibody fragment
• Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
• Reduces process time and amount of resin
• Suitable for process development and small-scale purification
• HiTrap^® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA^® system.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids