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Merck

GE17-0440-03

Con-A Sepharose 4B

Cytiva 17-0440-03, pack of 5 mL

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

concanavalin A (con A)

包裝

pack of 5 mL

製造商/商標名

Cytiva 17-0440-03

儲存條件

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

4-9

工作範圍

4-6

儲存溫度

2-8°C

一般說明

Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

應用

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids

特點和優勢

  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars.
  • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
  • Con A coupled to Sepharose 4B via cyanogen bromide activation

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

该页面展示了如何使用Cytiva的Tricorn™或XK色谱柱进行亲和层析色谱柱填充和制备。

This page shows how to prepare samples for purification with affinity chromatography.

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