跳转至内容
Merck

G9136

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

登录查看公司和协议定价


About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.75

product name

明胶 来源于猪皮肤, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

生物源

Porcine skin

品質等級

無菌

γ-irradiated

種類

Type A

產品線

BioXtra

形狀

lyophilized powder

包裝

pkg of 10 mg

技術

cell culture | mammalian: suitable

表面覆盖率

100‑200 μg/cm2

凝膠強度

~300 g Bloom

溶解度

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

運輸包裝

ambient

儲存溫度

room temp

正在寻找类似产品? 访问 产品对比指南

應用

建议将其按100-200 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用和研究目标。

明胶已用于多种应用。 它可用于对细胞培养器皿进行包被以改善细胞的附着,加入至PCR以稳定Taq DNA,作为Western blotting、ELISA和免疫化学中的封闭试剂,以及作为细菌学中用于物种分化培养基的组分。 作为一种生物相容性的聚合物,它已被作为递送载体用于活性生物分子的释放以及支架的生成用于组织工程应用。在制药工业中,明胶可用作悬浮和包封剂,以及其他应用。

成分

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶,当在室温下储存于密闭容器中时,将可保持多年不变。 当在空气存在时在100℃下加热时,它会溶胀变软,并分解成碳质物质并伴有吡啶碱和氨的释放。

準備報告

该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂,如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数,可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom指数变化于90至300 g之间。

本品为A型冻干粉,适用于细胞培养,凝胶强度为300。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Sonia Capellero et al.
International journal of molecular sciences, 23(2) (2022-01-22)
Peritoneal metastases are the leading cause of morbidity and mortality in ovarian cancer. Cancer cells float in peritoneal fluid, named ascites, together with a definitely higher number of non neo-neoplastic cells, as single cells or multicellular aggregates. The aim of
Giulia Villari et al.
The EMBO journal, 39(24), e103661-e103661 (2020-11-21)
Although subcellular positioning of endosomes significantly impacts on their functions, the molecular mechanisms governing the different steady-state distribution of early endosomes (EEs) and late endosomes (LEs)/lysosomes (LYs) in peripheral and perinuclear eukaryotic cell areas, respectively, are still unsolved. We unveil
Sandeep Kadekar et al.
Cellular and molecular bioengineering, 14(1), 121-130 (2021-02-27)
Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The
Marta Czernik et al.
Nature protocols, 11(11), 2170-2188 (2016-10-07)
This protocol describes how to convert the chromatin structure of sheep and mouse somatic cells into spermatid-like nuclei through the heterologous expression of the protamine 1 gene (Prm1). Furthermore, we also provide step-by-step instructions for somatic cell nuclear transfer (SCNT)
Carla Luana Dinardo et al.
PloS one, 10(12), e0145062-e0145062 (2015-12-15)
Recent evidence shows the rigidity of vascular smooth muscle cells (VSMC) contributes to vascular mechanics. Arterial rigidity is an independent cardiovascular risk factor whose associated modifications in VSMC viscoelasticity have never been investigated. This study's objective was to evaluate if

商品

培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

实验方案

该用于细胞培养应用的明胶包被实验方案提供了明胶类型、表面覆盖浓度和优化建议等相关信息。

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门