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Merck

G5154

Sigma-Aldrich

GMEM培养基

With sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

别名:

GMEM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

GMEM培养基, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

L-glutamine: no
phenol red: 0.0213 g/L
NaHCO3: 2.75 g/L
glucose: 4.5 g/L (Dextro)

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

Glasgow最低必需培养基是通过修改Eagle BME培养基而开发的。该培养基可用于研究影响细胞能力的遗传因素。
用于粘附肾细胞系,例如幼仓鼠肾细胞 (BHK)。

應用

Glasgow最低必需培养基已用于培养:
  • Hoxb7-绿色荧光蛋白(GFP)小鼠胚胎干(ES)细胞系,用于诱导输尿管芽分化
  • 小鼠E14 Tg2a胚胎干(mES)细胞
  • 转导中国仓鼠卵巢(CHO)细胞表达和产生Siglec-1-Fc融合蛋白

重構

补充 0.292 g/L L -谷氨酰胺和胰蛋白磷酸盐肉汤溶液(T8159)100 mL/L 的培养基。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Marco D'Addio et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(11), e22017-e22017 (2021-10-27)
Cellular interactions between endothelial cells and macrophages regulate macrophage localization and phenotype, but the mechanisms underlying these interactions are poorly understood. Here we explored the role of sialoglycans on lymphatic endothelial cells (LEC) in interactions with macrophage-expressed Siglec-1 (CD169). Lectin-binding
Sacha Robert et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known.
Cinzia Caprio et al.
Disease models & mechanisms (2021-02-21)
The Ezh2 gene encodes a histone methyltransferase of the Polycomb Repressive Complex 2 that methylates histone H3 lysine 27. In this work we asked whether EZH2 has a role in the development of the pharyngeal apparatus and whether it regulates
Marieke Aarts et al.
Genes & development, 31(20), 2085-2098 (2017-11-16)
Expression of the transcription factors OCT4, SOX2, KLF4, and cMYC (OSKM) reprograms somatic cells into induced pluripotent stem cells (iPSCs). Reprogramming is a slow and inefficient process, suggesting the presence of safeguarding mechanisms that counteract cell fate conversion. One such
Isabelle Re Nett et al.
EMBO reports, 19(8) (2018-06-14)
Mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signalling is implicated in initiation of embryonic stem (ES) cell differentiation. The pathway is subject to complex feedback regulation. Here, we examined the ERK-responsive phosphoproteome in ES cells and identified the negative regulator

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