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Merck

G2N10

Sigma-Aldrich

GenElute 植物基因组 DNA 微量制备试剂盒

greener alternative

sufficient for 10 purifications

别名:

植物基因组DNA少量制备, Gen Elute

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.55

用途

sufficient for 10 purifications

环保替代产品特性

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

储存温度

15-25°C

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一般描述

GenElute植物基因组DNA制备试剂盒可以简单、方便的从各种植物中分离高纯度的基因组DNA。该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起,同时无需采用昂贵的树脂、RNase处理,以及诸如苯酚和氯仿等有害化学成分。

首先在液氮中研磨植物组织,在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作,去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤,进一步纯化基因组DNA。

纯化所得的DNA可直接用于PCR、限制性酶切、克隆、测序和Southern印迹等下游应用。
GenElute植物基因组DNA小量制备试剂盒可以简单、方便的从各种植物中分离高纯度的DNA。GenElute试剂盒将硅胶膜系统的优势与微量离心形式结合在一起,同时无需采用昂贵的树脂、RNase处理,以及诸如苯酚和氯仿等有害化学成分。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 (The 12 Principles of Greener Chemistry),更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法,本产品符合固有安全化学原则(Inherently Safer Chemistry)。

应用

纯化所得的高纯度DNA可直接供以下敏感的下游应用使用:
  • PCR
  • 限制性内切酶酶切
  • 克隆
  • Southern印迹
GenElute Plant Genomic DNA Miniprep Kit has been used:
  • in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
  • in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
  • in genomic DNA isolation from leaves

特点和优势

  • 起始材料:不超过100 mg的植物组织
  • 预期得率:至多20 μg
  • 洗脱体积:100 - 200 μl
  • 所需时间:<40分钟
  • 是否需要RNase处理:否

原理

在1小时内,可从至多100 mg新鲜组织或10 mg冻干材料中提取出微克级的DNA。首先在液氮中研磨植物组织,在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作,去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤,进一步纯化基因组DNA。纯化所得DNA长度超过20 kb。

其他说明

更多信息,请见www.sigma-aldrich.com/genomicdna

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • C2112Column Preparation Solution化学品安全说明书

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

补充剂危害

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

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Phytohormone interplay controls proliferation of in vitro cultivated cells of Arabidopsis thaliana ethylene-insensitive mutants.
N S Stepanchenko et al.
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 442, 46-49 (2012-03-20)
Ellen Erzeel et al.
Plant molecular biology, 81(4-5), 401-415 (2013-01-19)
Lysine biosynthesis in plants is tightly regulated by feedback inhibition of the end product on the first enzyme of the lysine-specific branch, dihydrodipicolinate synthase (DHDPS). Three complete DHDPS coding sequences and one partial sequence were obtained in Medicago truncatula via
Stefanie P Glaeser et al.
International journal of systematic and evolutionary microbiology, 63(Pt 2), 521-525 (2012-04-24)
A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain E103(T), was isolated from the skin of the medical leech Hirudo verbana. 16S rRNA gene sequence analysis showed that the isolate was closely related to species of the genus Castellaniella. Castellaniella ginsengisoli DCY36(T)
Glomus hyderabadensis, a new species: its taxonomy and phylogenetic comparison with related species
Rani SS, et al.
Mycotaxon, 89(2), 245-254 (2004)
Peter Kämpfer et al.
International journal of systematic and evolutionary microbiology, 62(Pt 6), 1342-1347 (2011-08-11)
The taxonomy of strain CCUG 55240(T), a Gram-staining-positive, aerobic, endospore-forming bacterium that was isolated from a paper mill, was investigated using a polyphasic approach. In phylogenetic analysis based on 16S rRNA gene sequences, the novel strain was grouped with established

实验方案

本方案描述了使用GenElute植物基因组DNA Miniprep试剂盒,从多种植物中分离纯DNA的简单、便捷操作步骤。

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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