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NA2110

Sigma-Aldrich

GenElute™ 细菌基因组DNA试剂盒

sufficient for 70 purifications

别名:

细菌基因组DNA, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 70 purifications

技術

DNA purification: suitable

儲存溫度

15-25°C

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一般說明

GenElute 细菌基因组DNA试剂盒可以简单、方便地从革兰氏阴性菌中分离高纯度基因组DNA。对于大多数革兰氏阳性菌，本试剂盒必须结合选购的溶菌酶 (L4919)来有效裂解较厚的肽聚糖细胞壁。为方便您制备溶菌酶储液，我们提供了一种稀释的革兰氏阳性溶解溶液。

该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

應用

纯化所得的细菌基因组DNA可用于以下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 克隆

特點和優勢

  • 起始材料：不超过1.5 mL培养物
  • 预期得率：至多20 μg
  • 洗脱体积：400 μl
  • 所需时间：70 - 120分钟
  • A260/A280比率：1.6 - 1.9
  • 无需苯酚、氯仿或乙醇沉淀步骤

原則

细菌首先在含有离液盐的溶液之中裂解，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。

预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

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说明
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  • R6148RNase A solution化学品安全说明书

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訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system, Respiratory system

儲存類別代碼

3 - Flammable liquids

閃點（°F）

77.0 °F - closed cup

閃點（°C）

25 °C - closed cup

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Hyunjin Yoon et al.
Infection and immunity, 79(1), 360-368 (2010-11-03)
Salmonella enterica serovar Typhimurium is an intracellular pathogen and a main cause of food-borne illness. In this study, a quantitative PCR (qPCR)-based competitive index (CI) method was developed to simultaneously compare the growth of multiple Salmonella strains. This method was
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Steffen Porwollik et al.
Methods in molecular biology (Clifton, N.J.), 394, 89-103 (2008-03-28)
Microarray technology provides a convenient and relatively inexpensive way of investigating the genetic content of bacterial genomes by comparative genomic hybridization. In this method, genomic DNA of an unknown bacterial strain of interest and that of a closely related sequenced
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