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種類
Type VII
形狀
lyophilized powder
比活性
≥50 units/mg protein
分子量
34 kDa
運輸包裝
wet ice
儲存溫度
−20°C
相关类别
一般說明
嗜热脂肪芽孢杆菌磷酸果糖激酶(BsPFK)是一种可被磷酸烯醇丙酮酸(PEP)沿着一个二聚体-二聚体界面结合而变构抑制的同四聚体。
来自嗜热脂肪芽孢杆菌的果糖6-磷酸激酶是一种34 kDa的酶,属于磷酸果糖激酶(PFK)样超家族。位于活性位点的谷氨酸161和精氨酸162残基对于底物结合至关重要。
應用
来自嗜热脂肪芽孢杆菌的果糖-6-磷酸激酶已显示出可与神经元一氧化氮合酶(nNOS)相互作用,引起糖酵解代谢障碍和营养不良性肌肉的易疲劳性增加。
来自嗜热脂肪芽孢杆菌的果糖-6-磷酸激酶已被用于:
- 在C8位氘化的ATP(C8-D ATP)存在下对果糖磷酸激酶活性进行稳态分析
- 定量肌肉磷酸果糖激酶(PFK)活性的标准曲线生成
Fructose-6-phosphate Kinase from Bacillus stearothermophilus has been used in the assay mixture for mass spectrometry assay for phosphoglucoisomerase (PGI) (G6P to F6P reaction).
生化/生理作用
果糖-1,6-二磷酸酶(FBP)是葡萄糖代谢中一种重要的酶。它可催化果糖-1,6-双磷酸水解为果糖6-磷酸和无机磷酸。在糖酵解循环的限速步骤中,果糖-6-磷酸激酶会将果糖-6-磷酸转化为果糖1,6-双磷酸。
Phosphofructokinase (PFK) is an essential bifunctional enzyme that serves as a critical regulator in the intermediate stages of glycolysis. PFK is strongly linked to caveolae and is brought to caveolae by caveolin-1 in vascular smooth muscle cells (VSMCs).
單位定義
在pH 9.0、30 °C条件下,一单位在每分钟可将1.0 μm的果糖-6-磷酸和ATP转化为果糖1,6-二磷酸和ADP。
外觀
含缓冲盐的冻干粉末(如磷酸盐缓冲液或含NaCl的Tris缓冲液)
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
M D Wallace et al.
Oncogene, 33(28), 3688-3695 (2013-08-27)
Defective DNA replication can result in genomic instability, cancer and developmental defects. To understand the roles of DNA damage response (DDR) genes on carcinogenesis in mutants defective for core DNA replication components, we utilized the Mcm4(Chaos3/Chaos3) ('Chaos3') mouse model that
Analysis of the phosphofructokinase subunits and isoenzymes in human tissues
G.A. Dunaway et al.
The Biochemical Journal, 251, 755-757 (1988)
Gamut of glycolytic enzymes in vascular smooth muscle cell proliferation: Implications for vascular proliferative diseases
Sarkar A, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 167021-167021 (2024)
The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases
Kenyon CP, et al.
BMC Biochemistry, 12(1), 36-36 (2011)
[Pain treatment. Systematized education in epidural pain treatment].
I H Iversen
Sygeplejersken, 85(8), 24-31 (1985-02-20)
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