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一般說明
氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加,镁缺乏会导致产量降低。
應用
氯化镁溶液已用作以下材料的组分:
- 聚合酶链式反应(PCR)扩增混合液
- 简单序列重复(ISSR)-PCR扩增混合液
- 16S-23S rRNA基因内转录间隔区(ITS)PCR扩增及限制性内切分析(ITS-PCR- rflp)反应缓冲液
- 用于定量逆转录-聚合酶链反应(qRT PCR)的PCR反应混合物,以扩增逆转录cDNA
- 用于RT-PCR检测基因表达的反应混合物
適合性
适用于聚合酶链反应的优化。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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实验方案
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