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應用
分散酶I已被用于肺组织消化和流式染色处理,以及小鼠CD4细胞分离。该酶还被用于消化切割伤口及少量周围皮肤,用于GFP+(绿色荧光蛋白)细胞检测。本研究旨在探讨骨髓间充质干细胞的分化和血管生成对创面愈合的影响。它还被用于在小鼠真皮成纤维细胞分离过程中去除表皮。
适用于制备测序用单细胞悬液。
适用于制备测序用单细胞悬液。
分散酶I已被用于评估羊膜在愈合过程早期阶段对伤口大小的影响。 分散酶I还被用于研究以二酪氨酸为底物的蛋白酶测定。
生化/生理作用
分散酶I是一种快速、有效、温和的中性蛋白酶,可以将完整的表皮层与真皮层分开。它还可以将培养物中的完整上皮细胞层与下层分离。该酶可在消化基底膜区域的同时保留上皮细胞的活力。它还可用于防止悬浮培养物中的细胞结块。该蛋白酶可水解纤连蛋白和IV型胶原蛋白,但不能水解层粘连蛋白、V型胶原蛋白、血清白蛋白或转铁蛋白。它可以水解非极性氨基酸残基的N-末端肽键。它优先水解具有暴露疏水氨基酸残基的变性和细胞间蛋白质。Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ 和Al3+可以激活该酶活性。EDTA、EGTA、Hg2+ 和其他重金属可抑制该酶活性。每g-mol纯化分散酶I含有1g锌原子。如果通过EDTA或EGTA等螯合剂除去锌成分,则产生无活性的酶蛋白。该酶不受血清抑制。
單位定義
若无其他说明,则一个酶活性单位是指在pH7.5、37℃条件下每分钟水解酪蛋白并产生相当于1.0 μM (181 μg) 酪氨酸的颜色(使用Folin-Ciocalteu试剂显色)所需的酶量。
外觀
冻干粉末,含醋酸钙
法律資訊
Dispase is a registered trademark of Godo Shusei Co., Ltd.
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
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商品
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
相关内容
胶原酶指南。胶原酶是一种可分解将动物组织结合在一起的天然胶原的酶,由多种微生物和不同的动物细胞产生。
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
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