293 N3S

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human kidney
• 包装: tube of 5 μg 92052131-DNA-5UG; pkg of vial of cells 92052131-1VL
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Not specified
• 产品: Adenovirus 5 early region (E1) antigens
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human embryo kidney

细胞系描述

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培养基

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

传代培养常规

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.