Vero (AC-free)

8011101, African green monkey kidney, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: Vero (AC-free), from African green monkey kidney, 08011101, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 生物来源: African green monkey kidney
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 受体: Not specified

说明

一般描述

Vero cells are anchorage-dependent cells derived from the kidney of African green monkey cells. They have applications in molecular and cell biology research. Vero cells are a useful host for the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.

细胞系来源

African green monkey kidney cells, serum-free

细胞系描述

Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).

培养基

VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他说明

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