CHO-K1 Cell Line from hamster

85051005, hamster ovary, Epithelial

• 别名: CHO cell clone K1 Cells, CHOK1 Cells
• 分類程式碼代碼: 41106514

属性

• product name: CHO-K1 Cell Line from hamster, 85051005, from hamster ovary
• 生物源: hamster ovary
• 增長模式: Adherent
• 染色體組型: Hypodiploid, modal no. 20
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Hamster Chinese ovary

細胞系描述

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

應用

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

培養基

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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