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Merck

C4963

Sigma-Aldrich

过氧化氢酶-聚乙二醇

lyophilized powder, ~40,000 units/mg protein

别名:

PEG-过氧化氢酶

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About This Item

MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

~40,000 units/mg protein

分子量

PEG 5,000

成份

Protein, ~50% E405

標籤範圍

~40 mol PEG per mol protein

基質結合

secondary amine linkage.

儲存溫度

−20°C

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應用

经 PEG-过氧化氢酶处理的肺动脉内皮细胞可有效抑制 20-HETE(羟基二十碳四烯酸)诱导的荧光增强。但实验排除了 DCF(二氯荧光素)潜在的非特异性荧光。本实验研究了 20-HETE 对超氧化物产生和 NADPH 氧化酶活化的影响。

生化/生理作用

来自牛肝的过氧化氢酶催化 H 2 O 2 分解为水和氧。是一种四聚体,由分子量为 60 kDa 的四个相等的亚单位组成。每个亚基均含有与血红素 IX 基团结合的铁。该酶还能与靠近血红素基团的 NADP 强烈结合。过氧化氢酶活性在 pH 4.0-8.5 范围内恒定。pI 为 5.4。3-氨基-1-H-1,2,4 三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、联茴香胺和硝酸盐可抑制酶活性。用抗坏血酸或抗坏血酸/Cu 2 + 孵育过氧化氢酶会导致过氧化氢酶分子的降解。它不需要任何激活剂。

包裝

基于蛋白质含量的包装规格

其他說明

来自牛肝脏的过氧化氢酶偶联甲氧基-聚乙二醇。

外觀

含 PEG 加 5% 柠檬酸盐缓冲盐

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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