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表单
lyophilized powder
比活
100-300 units/mg protein
分子量
~175 kDa
组成
Protein, 65-85%
异质活性
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
储存温度
2-8°C
应用
在临床分析中，该酶可与肌酸酶、肌氨酸脱氢酶或肌氨酸氧化酶以及甲醛脱氢酶结合用于酶法测定肌酐。
来自微生物的肌酐酶可通过与其他酶共固定进行肌酐测定而用于安培生物传感器的制备。
生化/生理作用
来自假单胞菌属的肌酐酶是一种每个亚基分子量为28.4 kDa的同六聚体酶。它是一种环状酰胺水解酶，可催化肌酸酐向肌酸的可逆性转化。每个单体在β-链的C末端和主要α-螺旋的N末端附近都含有一个双核锌中心。这些锌离子指示着活性位点的位置。
物理属性
等电点： 4.7
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
米氏常数：3.2 x 10‾2M（肌苷），5.7 x 10‾2M（肌酸）
结构：每摩尔的酶6个亚基（每个亚基结合一摩尔的锌）
抑制剂：Ag+、Hg++、N-溴代琥珀酰亚胺、EDTA
最适pH值： 6.5 − 7.5
最适温度： 70°C
pH稳定性： pH 7.5 − 9.0 (5°C，16小时)
热稳定性： 低于70°C（pH 7.5，30分钟）
单位定义
在pH 8.0、25 °C条件下，一个单位将在每分钟内水解1.0 μmole的肌苷
外形
含有蔗糖和BSA作为稳定剂的冻干粉末
分析说明
通过缩二脲法测定的蛋白质。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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