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一般說明
考马斯亮蓝染色剂在十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS PAGE) 中被广泛用于蛋白质的检测。亮蓝 G 染色因其灵敏度接近银染法而被称为蓝银染色。考马斯亮蓝 G 染色液通过疏水作用与蛋白质结合。它可以有效染色角膜前囊膜，毒性相对较小。
應用
亮蓝 G-胶体浓缩液用于在分析酿酒酵母中的转录因子（TFIID）复合物前，进行体外胃蛋白酶消化后卵巢肿瘤液蛋白质组中
- 的蛋白质片段的染色
重構
用 800 mL 水稀释至 1 L 后，混悬液将含有 0.1% (w/v) 亮蓝 G、0.29 M 磷酸和 16% 饱和硫酸铵。
分析報告
已在 SDS-PAGE 上检测了本品的适用性。
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说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
8B - Non-combustible corrosive hazardous materials
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels
Dyballa N and Metzger S
Journal of Visualized Experiments (2009)
Purification of Active TFIID from Saccharomyces cerevisiae EXTENSIVE PROMOTER CONTACTS AND CO-ACTIVATOR FUNCTION
Auty R, et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004)
Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
Julie Ducreux et al.
Bioconjugate chemistry, 19(10), 2088-2094 (2008-09-24)
PEGylation of antibodies is known to increase their half-life in systemic circulation, but nothing is known regarding whether PEGylation can improve the inhibitory potency of antibodies against target receptors. In this paper, we have examined this question using antibodies directed
Antonis Ververis et al.
Cellular and molecular life sciences : CMLS, 73(1), 163-184 (2015-07-15)
Katanins are microtubule (MT)-severing AAA proteins with high phylogenetic conservation throughout the eukaryotes. They have been functionally implicated in processes requiring MT remodeling, such as spindle assembly in mitosis and meiosis, assembly/disassembly of flagella and cilia and neuronal morphogenesis. Here
实验方案
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
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