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Merck

76220

Sigma-Aldrich

木瓜蛋白酶 来源于番木瓜

powder, ≥3 U/mg

别名:

Papain powder

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
42030311
NACRES:
NA.54

生物源

papaya

形狀

powder

比活性

≥3 U/mg

分子量

Mr ~23000

儲存溫度

2-8°C

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應用

木瓜蛋白酶被用作嫩肉精。它还被用于在原代细胞培养物制备中分解组织。

生化/生理作用

木瓜蛋白酶是一种可切割肽键的半胱氨酸蛋白酶,并且已知可切割抗体的Fc部分。

單位定義

1个酶活力单位(U)相当于在pH 6.2和25℃下每分钟释放 1 μmol N-苯甲酰-L-精氨酸乙酯(BAEE,货号12880)所需的酶量

其他說明

一种重要的半胱氨酸蛋白酶 ;多肽合成

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Review: novel cysteine proteases of the papain family.
F Bühling et al.
Advances in experimental medicine and biology, 477, 241-254 (2000-06-13)
Catalytic mechanism in papain family of cysteine peptidases.
A C Storer et al.
Methods in enzymology, 244, 486-500 (1994-01-01)
M Y Gololobov et al.
Biotechnology and bioengineering, 40(3), 432-436 (1992-07-01)
The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters alpha and beta for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-LLeuNH(2) from Mal-L-Phe-L-AlaOMe and L-LeuNH(2). The
Adrienne McMahon
Journal of AOAC International, 99(1), 223-241 (2016-02-10)
The main objective of the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) project is to establish international consensus methods for infant formula and adult nutritionals, which will benefit intermarket supply and dispute resolution. A collaborative study was
Carolina Gomis Perez et al.
Science advances, 8(1), eabl4411-eabl4411 (2022-01-06)
Many cellular activities, such as cell migration, cell division, phagocytosis, and exo-endocytosis, generate and are regulated by membrane tension gradients. Membrane tension gradients drive membrane flows, but there is controversy over how rapidly plasma membrane flow can relax tension gradients.

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