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Merck

51558

Sigma-Aldrich

HEPES 缓冲盐溶液

BioUltra, for molecular biology, 2× concentrate

别名:

HEPES缓冲液

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About This Item

MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

產品線

BioUltra

形狀

liquid

品質

2× concentrate

成份

dextrose, 2.0 g/L
HEPES, 10 g/L
KCl, 0.74 g/L
NaCl, 16 g/L
Na2HPO4.2H2O, 0.27 g/L

濃度

2 ×

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

折射率

n20/D 1.339

pH值

7.2 (25 °C)

正離子痕跡

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

&lambda ;

neat

紫外吸收

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04

SMILES 字串

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI 密鑰

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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一般說明

HEPES 缓冲盐水是组织培养缓冲液,其用于维持细胞培养物的 pH。它是水溶性的,不依赖大气。HEPES 在氯化钠溶液中破坏镁。

應用

HEPES 缓冲盐水已被使用:
  • 用于制备质粒 DNA
  • 作为制备用于 DNA 构建载体的转染主混合物的试剂
  • 开发用于细胞渗漏测定的水凝胶前体

成分

浓度(溶于 H2O):10g/L HEPES;16g/L NaCl;0.74g/L KCl;0.27g/L Na2HPO4.2H2O;2.0g/L 右旋糖

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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C P Leamon et al.
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Current Protocols in Immunology, 10, 10-10 (2001)
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