推荐产品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human, mouse
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... TIAM1(7074)
一般說明
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.
Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
免疫原
Human
a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
應用
Immunoblotting (1:1000)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Highly Toxic (H)
外觀
In Tris-Citrate/phosphate, pH 7.0-8.0.
重構
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
分析報告
Positive Control
Jurkat or NIH3T3 cells
Jurkat or NIH3T3 cells
其他說明
Antibody should be titrated for optimal results in individual systems.
Mertens, A.E., et al. 2003. FEBS Lett.546, 11.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
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