SF006
ESGRO Complete 细胞消化液
The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.
别名:
Stem Cell Tested Accutase
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所有图片(1)
About This Item
分類程式碼代碼:
12352207
eCl@ss:
32160801
NACRES:
NA.75
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品質等級
形狀
liquid
製造商／商標名
Chemicon®
技術
cell culture | stem cell: suitable
輸入
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
運輸包裝
dry ice
一般說明
ESGRO Complete™ Accutase 是一种蛋白水解和胶原分解酶的细胞分离溶液，经确认可用于分离在无血清条件下用ESGRO Complete 克隆级培养基（目录号SF001-500）培养的小鼠胚胎干细胞。 Accutase 不含有哺乳动物或细菌来源产品。
應用
细胞分离：
1.将Accutase®在室温下解冻。
2.用无菌PBS洗涤孔板、烧瓶或珠子。
3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。
4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。
5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。
1.将Accutase®在室温下解冻。
2.用无菌PBS洗涤孔板、烧瓶或珠子。
3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。
4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。
5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。
外觀
冷冻无菌液体，即用型制剂。 每批都已经过无菌性（通过USP膜过滤方法）、酶活性（通过合成发色四肽测试）和细胞与组织培养塑料的分离性能测试。
1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。
1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。
儲存和穩定性
保存于-20°C可保持稳定。请参阅标签上的批次失效日期。 建议收货时保存于-20°C。 解冻后，Accutase® 可以在4°C下最多保存2个月。请勿在室温下存放。
法律資訊
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Darren A Cusanovich et al.
Cell, 174(5), 1309-1324 (2018-08-07)
We applied a combinatorial indexing assay, sci-ATAC-seq, to profile genome-wide chromatin accessibility in ∼100,000 single cells from 13 adult mouse tissues. We identify 85 distinct patterns of chromatin accessibility, most of which can be assigned to cell types, and ∼400,000
Lina Dahl et al.
PloS one, 3(4), e2025-e2025 (2008-04-24)
The molecular mechanisms regulating the expansion of the hematopoietic system including hematopoietic stem cells (HSCs) in the fetal liver during embryonic development are largely unknown. The LIM-homeobox gene Lhx2 is a candidate regulator of fetal hematopoiesis since it is expressed
Na Suo et al.
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Thangaselvam Muthusamy et al.
Stem cell reports, 3(1), 169-184 (2014-07-30)
We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of
Elena Vezzoli et al.
The Journal of clinical investigation, 129(6), 2390-2403 (2019-05-08)
A disintegrine and metalloproteinase 10 (ADAM10) is implicated in synaptic function through its interaction with postsynaptic receptors and adhesion molecules. Here, we report that levels of active ADAM10 are increased in Huntington's disease (HD) mouse cortices and striata and in
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