推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
monoclonal
种属反应性(根据同源性预测)
all
包装
antibody small pack of 25 μL
技术
western blot: suitable
同位素/亚型
IgG1κ
靶向翻译后修饰
unmodified
一般描述
Anti-RAINBOW Antibody, Cat. No. MABC1690-25UL, validated for use in Western Blotting.
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).
特异性
Anti-RAINBOW is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.
免疫原
Remazol-dyes phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).
应用
Anti-RAINBOW, Cat. No. MABC1690, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Western Blotting Analysis: A representative lot detected Remazol-dye stained protein markers in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).
Western Blotting Analysis: A 1:250 and 1:500 dilution from a representative lot detected Remazol-dye Pre-Stained protein standards.
Western Blotting Analysis: A 1:250 and 1:500 dilution from a representative lot detected Remazol-dye Pre-Stained protein standards.
质量
Evaluated by Western Blotting in Pre-Stained protein standard. Western Blotting Analysis: A 1:1,000, 1:5,000, and 1:10,000 dilution of this antibody detected Remzaol-dye prestained protein standards .
外形
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl, pH 7.4, with 0.05% sodium azide.
其他说明
Concentration: Please refer to lot specific datasheet.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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