ABS1631

Anti-phospho-eNOS (Ser602)

from rabbit

• 别名: Nitric oxide synthase endothelial, Constitutive NOS, EC-NOS, eNOS, NOS III, NOS type III
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: bovine
• 物種活性（以同源性預測）: human (based on 100% sequence homology)
• 技術: ELISA: suitable; western blot: suitable
• NCBI登錄號: NP_851380
• UniProt登錄號: P29473
• 運輸包裝: ambient
• 目標翻譯後修改: phosphorylation (pSer602)
• 基因資訊: mouse ... Nos3(287024)

说明

一般說明

Nitric oxide synthase, endothelial (UniProt: P29473; also known as EC:1.14.13.39; constitutive NOS, EC-NOS, eNOS, NOS III, NOS type III) is encoded by the NOS3 gene (Gene ID: 287024) in bovine. The eNOS is mainly expressed in vascular endothelial cells and cardiomyocytes. It produces nitric oxide (NO) that is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. In endothelial cells, eNOS is localized in specialized plasmalemmal pockets known as caveolae, which contain a scaffolding protein termed caveolin. Acylation of eNOS by either myristate or palmitate targets it to caveolae, which facilitates enzyme activation following receptor stimulation. In a resting cell, eNOS is inhibited by its interaction with caveolin. Following agonist activation, eNOS dissociates from caveolin and translocates to the cytosol. The eNOS is generally activated in response to an increase in intracellular Ca2+ following stimulation with receptor-dependent agonists such as acetylcholine. However, shear stress can also activate this enzyme independently of Ca2+ level changes, via a tyrosine kinase-dependent mechanism involving its phosphorylation and redistribution. Perturbations of eNOS phosphorylation have been reported in a number of diseases. The primary sites where eNOS gets phosphorylated are multiple serine residues and, to a lesser extent, on tyrosine and threonine residues. Ser 602 is reported to lie at the N-terminal edge of the 42-residue eNOS autoinhibitory element and is phosphorylated by ERK. Low concentrations of nitric oxide in the endothelial cells prevent their apoptotic death and maintain their integrity during inflammation and atherosclerosis. Distorted production of nitric oxide in endothelial cells has been linked to hypertension, hypercholesterolemia, diabetes, and even cardiac failure. (Ref.: Rafikov, R., et al. (2011). J. Endocrinol. 210(3): 271-284).

特異性

This polyclonal antibody specifically detects the phospho-Ser602 in eNOS. It targets a sequence of 11 amino acids surrounding Ser602.

免疫原

A linear peptide of 11 amino acids from the N-terminal edge of the 42-residue bovine eNOS autoinhibitory element.

應用

Detect endothelial Nitric oxide synthase using this rabbit polyclonal Anti-phospho-eNOS (Ser602), Cat. No. ABS1631. It has been tested for use in ELISA and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected phospho-eNOS (Ser602) in bovine aortic endothelial cell lysate (BAEC) (Salerno, J.C. et. al. (2014). Biosci Rep. 34(5). pii: e00137).

Western Blotting Analysis: A 1:250-1,000 dilution from a representative lot detected phospho-eNOS (Ser602) in bovine aortic endothelial cell lysates (Courtesy of Dr. Carol Chrestensen at Kennesaw State University, Kennesaw, GA).

ELISA Analysis: A 1:64,000 dilution from a representative lot detected phospho-eNOS (Ser602) in Phospho-602 peptide/Non-Phospho-602 peptide (Courtesy of Dr. Carol Chrestensen at Kennesaw State University, Kennesaw, GA).

品質

Evaluated by Western Blotting in bovine aortic endothelial cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-eNOS (Ser602) in 10 µg of bovine aortic endothelial cell lysate.

標靶描述

~150 kDa observed; 133.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2