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生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat, mouse
製造商/商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
mouse ... Cacng2(12300)
rat ... Cacng2(84347)
特異性
Stargazin [Stargazin样TARP(跨膜AMPA受体调节蛋白)]。 通过蛋白质印迹法,该抗体可识别大鼠脑Triton可溶性部分~ 35kda和~ 55kda的条带。该抗体主要与TARP γ -8反应,与γ -3和γ -4反应弱。Stargazin在海马体和小脑中强烈表达。
免疫原
来自大鼠细胞质结构域Stargazin的GST融合蛋白。
應用
研究子类别
突触&突触生物学
突触&突触生物学
研究类别
神经科学
神经科学
蛋白质印迹:0.1 μg/mL,大鼠脑的Triton可溶性部分。建议的稀释缓冲液是TBS-T,封闭缓冲液是3%脱脂牛奶。建议培养时间为室温下3小时过夜。
免疫组化:0.25-0.5 μg/mL,4%多聚甲醛固定组织。建议的稀释缓冲液是PBS-T。建议的透化方法是0.1% Triton X-100。建议培养时间为室温下2小时过夜。
免疫细胞化学
免疫沉淀:1-2 μg。建议反应容积为200-1000 μL。建议总蛋白浓度为2 mg/mL。建议培养时间为2-8°C下2小时。建议捕捉剂为蛋白A。
最佳工作稀释度必须由最终用户进行确定。
免疫组化:0.25-0.5 μg/mL,4%多聚甲醛固定组织。建议的稀释缓冲液是PBS-T。建议的透化方法是0.1% Triton X-100。建议培养时间为室温下2小时过夜。
免疫细胞化学
免疫沉淀:1-2 μg。建议反应容积为200-1000 μL。建议总蛋白浓度为2 mg/mL。建议培养时间为2-8°C下2小时。建议捕捉剂为蛋白A。
最佳工作稀释度必须由最终用户进行确定。
该抗Stargazin抗体经验证可用于IP、WB、IC、IH中Stargazin的检测。
外觀
免疫亲和纯化
形式:纯化
液体形式,含130 mM Tris-HCl、87 mM甘氨酸,pH 7.4。
儲存和穩定性
接收后,以未稀释的等分试样在-80°C下保存长达6个月。应避免反复冻融循环。解冻后抗体可在2-8°C下保存1-3周。
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk.
The Journal of Biological Chemistry null
Cell reports, 32(3), 107921-107921 (2020-07-23)
The anterior thalamus (AT) is critical for memory formation, processing navigational information, and seizure initiation. However, the molecular mechanisms that regulate synaptic function of AT neurons remain largely unexplored. We report that AMPA receptor auxiliary subunit GSG1L controls short-term plasticity
Proceedings of the National Academy of Sciences of the United States of America, 113(38), E5645-E5654 (2016-09-08)
AMPA-type glutamate receptors (AMPARs) mediate fast excitatory neurotransmission and predominantly assemble as heterotetramers in the brain. Recently, the crystal structures of homotetrameric GluA2 demonstrated that AMPARs are assembled with two pairs of conformationally distinct subunits, in a dimer of dimers
Neuropharmacology, 77, 28-38 (2013-09-17)
Behavioral sensitization to cocaine is associated with increased AMPA receptor (AMPAR) surface expression in the nucleus accumbens (NAc). This upregulation is withdrawal-dependent, as it is not detected on withdrawal day (WD) 1, but is observed on WD7-21. Its underlying mechanisms
Heliyon, 7(7), e07456-e07456 (2021-07-24)
Doxorubicin (Dox) is a chemotherapeutic agent used widely to treat a variety of malignant cancers. However, Dox chemotherapy is associated with several adverse effects, including "chemobrain," the observation that cancer patients exhibit through learning and memory difficulties extending even beyond
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