AB5927
Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3
Chemicon®, from guinea pig
别名:
ASIC3, Amiloride-sensitive Cation Channel 3, TNaC1, Testis Sodium Channel 1, Dorsal Root Ganglion ASIC (DRASIC)
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所有图片(1)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物源
guinea pig
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
特異性
ASIC3 (DRASIC)
免疫原
Epitope: 285-304 of extracellular domain of rat ASIC3
Synthetic peptide from the extracellular domain of rat ASIC3 (Catalog Number AG366). (C)TASLDPDDFDPEPSDPLGSP
應用
Detect Acid Sensitive Ion Channel 3 using this Anti-Acid Sensitive Ion Channel 3 Antibody, 285-304 of extracellular domain of rat ASIC3 validated for use in IC, IH.
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5927
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Immunocytochemistry: 1:100
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5927
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5927. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuroinflammation & Pain
Ion Channels & Transporters
Neuroinflammation & Pain
外觀
Affinity purified immunoglobulin. Liquid in PBS, pH 7.4 with 1% BSA and 0.05% sodium azide.
儲存和穩定性
Maintain at -20°C in undiluted for up to 6 months. Avoid repeated freeze/thaw cycles.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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