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AB1252

Sigma-Aldrich

抗细胞色素P450 酶CYP2E1抗体

serum, Chemicon®

别名:

cytochrome P450 2E1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenase

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

rat, human, mouse

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CYP2E1(1571)
mouse ... Cyp2E1(13106)
rat ... Cyp2E1(25086)

一般說明

细胞色素P450 (CYP)是一个巨大的、多样化的血红素蛋白超家族。它可以将脂肪酸花生四烯酸(AA)代谢为血管紧张度的调节因子。
大多数CYP可以代谢多种底物,并且可以催化多种反应。这种作用导致它们在诸多内源性和外源性分子的代谢中极为重要。在肝脏中,这些底物包括药物和毒性化合物,以及代谢产物,如胆红素(血红蛋白的分解产物)。细胞色素P450 酶存在于机体的许多其它组织中,包括胃肠道的粘膜,并在激素合成和分解(包括雌激素和睾丸素合成和代谢)、胆固醇合成和维生素D代谢中起重要作用。自2007年9月以来,我们已经知道了7700多种不同的CYP序列。

特異性

与肝脏微粒体部分(microsomal fraction)中的人和大鼠细胞色素P450 酶CYP2E1反应。与这些物种中的其它细胞色素P450 酶没有交叉反应。

應用

免疫组化:
一个先前批次被用于甲醛处理的冷冻切片。

最佳的工作稀释度必须由最终用户确定。
抗细胞色素P450 酶CYP2E1抗体是针对细胞色素P450 酶CYP2E1的抗体,可用于IH & WB。

品質

通过蛋白质印迹对人脑裂解物进行了评估。

蛋白质印迹分析:
该抗体的1:1000稀释液在10 μg小人脑裂解物中检测到细胞色素P450 酶CYP2E1。

標靶描述

57kda

外觀

不含防腐剂的缓冲液中的兔多克隆抗血清。

分析報告

对照
肝脏组织。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Rebecca L McCullough et al.
Molecular immunology, 75, 122-132 (2016-06-10)
Complement is implicated in the development of alcoholic liver disease. C3 and C5 contribute to ethanol-induced liver injury; however, the role of C5a receptor (C5aR) on myeloid and non-myeloid cells to progression of injury is not known. C57BL/6 (WT), global
Kyle J Thompson et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 52(6), 629-637 (2017-10-17)
This study sought to compare mice bred to preferentially consume high amounts of alcohol (crossed-high alcohol preferring, cHAP) to c57BL/6 (C57) mice using a chronic-binge ethanol ingestion model to induce alcoholic liver disease (ALD). Male C57 and cHAP mice were
Xiaoxia Jin et al.
Frontiers in pharmacology, 9, 1317-1317 (2018-12-14)
This study was designed to explore the role of cytochrome P4502E1 (CYP2E1) expression in the course of brain edema induced by subacute poisoning with 1,2-dichloroethane (1,2-DCE). Mice were randomly divided into five groups: the control group, the 1,2-DCE poisoned group
Murali Ganesan et al.
Cellular and molecular gastroenterology and hepatology, 5(2), 101-112 (2018-04-26)
Alcohol-induced progression of hepatitis C virus (HCV) infection is related to dysfunction of innate immunity in hepatocytes. Endogenously produced interferon (IFN)α induces activation of interferon-stimulated genes (ISGs) via triggering of the Janus kinase-signal transducer and activator of transcription 1 (STAT1)
Pengcheng Wang et al.
Biochemical pharmacology, 145, 218-225 (2017-09-11)
Acetylation is the major metabolic pathway of isoniazid (INH) mediated by N-acetyltransferases (NATs). Previous reports suggest that slow acetylators have higher risks of INH hepatotoxicity than rapid acetylators, but the detailed mechanisms remain elusive. The current study used Nat1/2(-/-) mice

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