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Merck
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文件

539480

Sigma-Aldrich

蛋白酶 K 来源于林伯氏白色念球菌

别名:

肽链内切酶

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About This Item

CAS号:
39450-01-6
EC 号:
3.4.21.14 (BRENDA, IUBMB)
EC 号:
3.4.21.64 (BRENDA, IUBMB)
MDL號碼:
MFCD00132129
分類程式碼代碼:
12352202
NACRES:
NA.21

形狀

lyophilized solid

品質等級

400

比活性

≥30 mAnson units/mg dry wt
≥40 mAnson units/mg protein

分子量

28.93 kDa

製造商／商標名

Calbiochem®

儲存條件

OK to freeze

顏色

white

溶解度

50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0: soluble

異物活動

DNase, none detected (nicking activity with pBR322, incubation for 6 h at 37°C)
RNase, none detected (ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C)

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

丝氨酸蛋白酶，对多种变性和天然的高分子量蛋白质具有很强的蛋白水解活性。它可使内源性核酸酶迅速蛋白水解失活，因此可用于分离mRNA和高分子量DNA。优先裂解N-取代疏水性、脂肪族和芳香族氨基酸羰基旁的键。可被DFP、Hg2+和PMSF抑制。不会被金属螯合剂、巯基试剂、TLCK或TPCK灭活。

應用

在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

生化／生理作用

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

警告

毒性：刺激性（B）

單位定義

一个mAnson单位定义为在37°C、pH 7.5条件下每分钟释放1.0 µM Folin阳性氨基酸（以酪氨酸计）的酶的量。

外觀

由乙酸钙冻干

重構

复溶后，储存在冰箱中（4°C）。用50 mM Tris-HCl、2 mM乙酸钙制备的pH 8.0的储备溶液在4°C下可稳定保存1年。

其他說明

Watazu, Y., et al. 1993.J. Lab. Clin. Anal.7, 81.
Lebherz, H.G., et al. 1986.生物化学。J.233, 51.
Ebeling, W., et al. 1974.Eur. J. Biochem.47, 91.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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