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Merck

P8038

Sigma-Aldrich

细菌蛋白酶

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

别名:

蛋白酶 来源于地衣芽孢杆菌, 枯草肽酶A, 碱性蛋白酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

種類

Type XXIV

形狀

lyophilized powder

比活性

≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid

分子量

27 kDa

純化經由

crystallization

運輸包裝

wet ice

儲存溫度

−20°C

特異性

枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白,并在碱性条件下具有活性。

應用

来自sigma的该酶已用于优化大鼠心脏均质化心室组织中所有线粒体群的释放。它还用于福尔马林固定石蜡包埋肝脏样本的预杂交处理,用于人和病毒DNA的检测。
这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶,并在碱性条件下具有活性。 产品P8038,也被称为枯草杆菌蛋白酶Carlsberg,已用于水解心肌细胞以研究心脏线粒体NHE1的沉默

生化/生理作用

蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

物理性質

枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。

單位定義

在pH 7.5,37 °C 条件下,一单位的酶每分钟将会水解酪蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色(Folin-Ciocalteu试剂提供的颜色)。

分析報告

氨基酸分析和等电聚焦电泳与枯草杆菌蛋白酶Carlsberg一致。

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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