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一般說明
在人 T 细胞和转染了人 CD28 的 BHK 细胞上识别 CD28。
小鼠抗CD28单克隆抗体(克隆ANC28.1 / 5D10)识别人T细胞上的CD28 & 人CD28转染的BHK细胞。 已针对冰冻切片的FC,IP,IHC和T细胞共刺激进行了验证。
蛋白 A 纯化的小鼠单克隆抗体。识别 CD28 蛋白。
免疫原
人
人 CD28 Ig 融合蛋白和 HPB-MLT 人肿瘤细胞
應用
流式细胞术(5 µg/ml)
冷冻切片(参见评论)
免疫沉淀法(参见评论)
T 细胞共刺激(参见评论)
冷冻切片(参见评论)
免疫沉淀法(参见评论)
T 细胞共刺激(参见评论)
包裝
请参考特定浓度批号的标签。
警告
毒性:标准处理(A)
外觀
在 150 mM NaCl,100 mM KCl,50 mM 磷酸钠中,pH 7.5。
重構
初次使用后,分装冻存(-20°C)以进行长期储存。避免冻/融循环。
其他說明
Kariv, I., et al. 1996.J. Immunol.157, 29.
61st Forum in Immunology.1995.Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G.1993.Curr.Opin.Immunol.5, 361.
61st Forum in Immunology.1995.Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G.1993.Curr.Opin.Immunol.5, 361.
单体和交联抗体会刺激 CD28+ 细胞表达 IL-2。使用 5 µg/ml 的抗 CD28 抗体和 FITC 偶联的山羊抗小鼠 IgG 对 5x105 培养的 HPB-MLT 细胞进行 FACS。也有报道表明该抗体可用于冷冻切片和免疫沉淀。合适的工作稀释度由与测定条件相关的变量决定。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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