14-429-M

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• 别名: CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
• UNSPSC代码: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

属性

• 生物来源: human
• 质量水平: 100
• 重组: expressed in E. coli
• 产品线: Upstate^®
• 表单: liquid
• 比活: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 保质期: 1 yr
• 分子量: M_w 71 kDa
• 纯化方式: (Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)
• 种属反应性: human
• 制造商/商品名称: Upstate^®
• 技术: activity assay: suitable (kinase)
• 溶解性: soluble
• 登记号: (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. ); NM_002755.2
• NCBI登记号: NM_002755.2
• UniProt登记号: Q02750
• 储存温度: −70°C
• 基因信息: human ... MAP2K1(5604)

说明

一般描述

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE

N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

生化/生理作用

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

包装

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

质量

routinely evaluated by phosphorylation of MBP

目标描述

MEK1

外形

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

储存及稳定性

1 year at -70°C

其他说明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形图: GHS07
• 警示用语：: Warning
• 危险声明: H317
• 预防措施声明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危险分类: Skin Sens. 1
• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(°C): Not applicable